人寡聚蛋白(oligomeric)ELISA試劑盒標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn),。若不能 馬上進(jìn)行試驗(yàn),，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融 

2．不能檢測(cè)含 NaN3 的樣品,，因 NaN3 抑制辣根過(guò)氧化物酶的（HRP）活性。 

3．組織處理：切割標(biāo)本后,，稱(chēng)取重量,。加入一定量的 PBS,，PH7.4。用液氮迅速冷凍 保存?zhèn)溆?。?biāo)本融化后仍然保持 2-8℃的溫度,。加入一定量的 PBS（PH7.4），用手 工或勻漿器將標(biāo)本勻漿充分,。離心 20 分鐘左右（轉(zhuǎn)/分）,。仔細(xì)收集上清。 分裝后一份待檢測(cè),，其余冷凍備用,。

人寡聚蛋白(oligomeric)ELISA試劑盒常見(jiàn)問(wèn)題解答：?jiǎn)枺何铱梢允褂迷噭┖姓f(shuō)明書(shū)中不推薦的樣本類(lèi)型嗎？答：我司試劑盒已針對(duì)試劑盒說(shuō)明書(shū)中列出的樣本類(lèi)型進(jìn)行了驗(yàn)證,。除已驗(yàn)證的樣本類(lèi)型外,，其他樣本類(lèi)型尚未進(jìn)行測(cè)試。有關(guān)更多信息可聯(lián)系我司技術(shù)服務(wù)部門(mén)為您解答,。Q:Can I use a sample type that is not recommended in the kit insert?A:The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.問(wèn)：我的樣本生成的值超出了分析的動(dòng)態(tài)范圍,，我能用這些樣本值么？答：建議僅使用標(biāo)準(zhǔn)曲線范圍內(nèi)的樣本值,。標(biāo)準(zhǔn)曲線范圍之外的值通常是非線性的,，這可能會(huì)導(dǎo)致錯(cuò)誤的外推值。產(chǎn)生高于zui高標(biāo)準(zhǔn)值的樣品應(yīng)（進(jìn)一步）稀釋并重復(fù)測(cè)定,。如果樣品低于測(cè)定范圍,，則認(rèn)為樣品不可檢測(cè)。Q:My samples generated values that were outside the dynamic range of the assay. Can I use these values?A:It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted and the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable.